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常用的荧光探针+滤色片参数

时间:2019/12/24 10:24:52   作者:郑士利   来源:正势利   阅读:143   评论:0
内容摘要:名称主要用途激发发射DAPIDNA,细胞核372456Hoechst33258染色体Q带,DNA合成365465CFP蛋白质示踪,荧光共振433476FITC免疫荧光490525CY2免疫荧光492510AlexaFluor488检测F/G肌动蛋白495517TexasRed线料体示踪595615MitoTracker...

名称

主要用途

激发

发射

DAPI

DNA,细胞核

372

456

Hoechst 33258

染色体Q带,DNA合成

365

465

CFP

蛋白质示踪,荧光共振

433

476

FITC

免疫荧光

490

525

CY2

免疫荧光

492

510

Alexa Fluor 488

检测F/G肌动蛋白

495

517

Texas Red

线料体示踪

595

615

Mito Tracker Red

溶酶体、线料体示踪

577

599

TRITC

蛋白质、单/多糖示踪

541

572

GFP

活细胞、蛋白质表达

479

509

Rhodamine 123

溶酶体、有丝分裂

505

534

YFP

蛋白质示踪,荧光共振

514

527


CHROMA带通滤色块
B1 39002 FITERS TYPE AOI https://cn.chroma.com/products/sets/39002-at-egfp-fitc-cy2-alexafluor-488
AT480/30x EX
AT505DC BS 45°
AT535/40m EM
G1 39002 FITERS TYPE AOI https://cn.chroma.com/products/sets/39010-at-texas-red-mcherry-alexafluor-594
AT560/40x EX
AT600DC BS 45°
AT635/40m EM
UV1 39000 FITERS TYPE AOI https://cn.chroma.com/products/sets/39000-at-dapi-hoechst-alexafluor-350
AT375/28x EX
AT415DC BS 45°
AT460/50m EM
B5 49303 - ET - Green#1 FISH FITERS TYPE AOI https://cn.chroma.com/products/sets/49303-et-green1-fish
ET495/25x EX
T515lp BS 45°
ET537/29m EM
G4 49305 - ET - Orange#1 FISH FITERS TYPE AOI https://cn.chroma.com/products/sets/49305-et-orange1-fish
ET546/22x EX
T565lpxr BS 45°
ET590/33m EM
V3 49302 - ET- Aqua FISH FITERS TYPE AOI https://cn.chroma.com/products/sets/49302-et-aqua-fish
ET436/20x EX
T455lp BS 45°
ET480/30m EM


CHROMA长通滤色块
B2 19002 - AT - GFP/FITC Longpass FITERS TYPE AOI https://cn.chroma.com/products/sets/19002-at-gfp-fitc-longpass
AT480/30x EX
AT505DC BS 45°
AT515lp EM
G2 19004 - AT - TRITC/Cy3 Longpass FITERS TYPE AOI https://cn.chroma.com/products/sets/19004-at-tritc-cy3-longpass
AT540/25x EX
AT565DC BS 45°
AT575lp EM
UV2 19000 - AT – UV/DAPI Longpass FITERS TYPE AOI https://cn.chroma.com/products/sets/19000-at-uv-dapi-longpass
AT375/28x EX
AT415DC BS 45°
AT435lp EM
V2 19011 - AT – Coumarin/Pacific Blue Longpass FITERS TYPE AOI https://cn.chroma.com/products/sets/19011-at-coumarin-pacific-blue-longpass
AT405/30x EX
AT440DC BS 45°
AT450lp EM
UV4 19011 - AT – Coumarin/Pacific Blue Longpass FITERS TYPE AOI
CT365/10X EX
T400LP BS 45°
ET420lp EM
B4 41015 - wtGFP Longpass Emission FITERS TYPE AOI https://cn.chroma.com/products/sets/41015-wtgfp-longpass-emission
AT405/30x EX
AT440DC BS 45°
AT450lp EM

 

荧光切片:https://www.thermofisher.com/order/catalog/product/F36924?SID=srch-srp-F36924












 




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